Genes CATA1-CATA4 expression in different sunflower hybrids at the pre-attachment phase of interaction with Orobanche cumana
DOI:
https://doi.org/10.52673/18570461.25.4-79.0Keywords:
catalase, sunflower, host-parasite interaction, broomrape, qPCR, oxidative stressAbstract
The transcriptional response of genes encoding isoforms of catalase (CATA1–CATA4) was investigated in the roots of two F1 sunflower hybrids with genetic resistance to Orobanche cumana (Favorit and P64LE20) and a susceptible hybrid (Performer), exposed to biotic stress during the pre-attachment phase of the host-parasite interaction. A comparative analysis of gene expression (qPCR), conducted both under optimal cultivation conditions and in the presence of biotic stress induced by germinated parasite seeds, at different time intervals (2, 6, 12, 24 hours post-inoculation), revealed distinct expression profiles. In the case of the F1 hybrid Favorit, an early and dynamic response was observed, characterized by successive activations and inhibitions of catalase gene expression, indicative of an effective adaptive strategy for antioxidant defense. The second resistant hybrid, P64LE20, exhibited a more delayed response, but it was more moderate and synchronized compared to Favorit, suggesting the presence of genetic variations in the mechanisms regulating resistance to O. cumana. In the case of the susceptible hybrid Performer, a biphasic response was observed, characterized by an excessively strong activation of gene expression in the first hours, followed by strong repression at 24 hours after co-cultivation with the pathogen, indicating an imbalance in antioxidant mechanisms and a reduced defensive capacity.
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